Establishment of a Primary Epididymal Cell Model From Epididymal Samples to Study CFTR Gene Regulation

Sponsor
University Hospital, Brest
Study ID
NCT06045702
Status
Recruiting
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Conditions

  • Congenital Bilateral Absence of Vas Deferens
  • Cystic Fibrosis
  • Sterility, Male

Eligibility Criteria

Sex
MALE
Age
18 Years - N/A
Healthy Volunteers
Accepted

Interventions

  • Epididymal sample — PROCEDURE
    During a planned surgical procedure carried out by the Urology and Medically Assisted Reproduction (MAP) department at Brest University Hospital in the patients included in the study, a remnant epididymal sample (between ½ and ¼ of a cc) will be kept for research purposes. These patients suffer from male infertility, leading them to undergo surgery in order to check for the presence of spermatozoa in the epididymis, and to recover spermatozoa which will be frozen and flaked in the AMP department of the CHU Brest, in the event of future procreation by in vitro fertilisation.

Study Details

The aim of this observational study is to better understand the role and involvement of the regulatory elements of the CFTR gene, with the aim of better describing the 3D organisation of chromatin at the CFTR locus in epididymal cells in patients with male infertility of any kind, or with cystic fibrosis or bilateral agenesis of the vas deferens, requiring scheduled surgery. The main questions it aims to answer are: * to better characterise this 3D organisation of the CFTR locus, the study of regulatory elements in primary epididymal cells is the most relevant and realistic model. * to gain a better understanding of the regulation of the CFTR gene in epididymal cells in order to gain a better understanding of the pathology of male infertility caused by bilateral agenesis of the vas deferens, a symptom and also a borderline form of cystic fibrosis. Participants will Epididymal samples will be taken by a urologist for the AMP department during the planned surgery. The rest of the samples taken will be recovered for research purposes, with the aim of recovering the epididymal cells contained in the sample. This is in no way an additional procedure and will have no impact on the patient's health..

Key Dates

First listed
Sep 21, 2023
Start date
Sep 11, 2024
Status verified
Mar 2026
Primary completion
Sep 9, 2026
Completion
Sep 11, 2026

Study Design

Enrollment
20 participants (estimated)

Arms

  • Arm: Group control
    Adult patients with no genetic pathology other than male infertility of any type leading to an inability to procreate without medical assistance, and requiring surgery to recover spermatozoa for possible in vitro fertilization.
  • Arm: CFTR-associated pathology
    Major patients with cystic fibrosis or male infertility due to agenesis of the vas deferens. These patients will be included in the study because they have the prospect of procreation by in vitro fertilisation requiring surgery to recover spermatozoa.

Primary Outcome Measure

3D organisation of chromatin at the CFTR locus [ Time Frame: 2 years ]

Central Contacts

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