Ultra-rapid Blastocyst Vitrification: Pilot Randomized Study

Sponsor
Fundacion Dexeus
Study ID
NCT07556601
Status
Recruiting
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Conditions

  • Infertility

Eligibility Criteria

Sex
FEMALE
Age
18 Years - 50 Years
Healthy Volunteers
Not accepted

Interventions

  • Standard Vitrification — OTHER
    1. Equilibration step: Blastocysts are equilibrated in Equilibration Solution (ES) for 10-12 minutes at room temperature. 2. Vitrification solution exposure: Blastocysts are then transferred to Vitrification Solution (VS) for a maximum exposure time of 90 seconds. 3. Loading: The blastocyst is loaded onto a Cryotop device (Kitazato). 4. Vitrification: The Cryotop is plunged directly into liquid nitrogen within 1 second of loading to ensure ultra-rapid cooling. 5. Capping: The Cryotop is immediately capped under liquid nitrogen conditions.
  • Ultra-Rapid Vitrification — OTHER
    The ultra-rapid vitrification protocol is identical to the standard protocol in terms of media, devices, and laboratory conditions, with the following modifications: Preparation step - Blastocyst shrinkage: Prior to equilibration, artificial collapse of the blastocyst is performed using a single laser pulse to induce blastocoel shrinkage. The subsequent steps are: 1. Equilibration step: Blastocysts are equilibrated in Equilibration Solution (ES) for 2-4 minutes. 2. Vitrification solution exposure: Transfer to Vitrification Solution (VS) for a maximum of 90 seconds. 3. Loading: Loading of the blastocyst onto a Cryotop device. 4. Vitrification: Immediate plunging of the Cryotop into liquid nitrogen within 1 second. 5. Capping: Immediate capping under liquid nitrogen.

Study Details

Blastocyst vitrification is standard in assisted reproduction, but clinical data on ultra-rapid vitrification are limited. This pilot study evaluates the safety, feasibility, and preliminary clinical performance of an ultra-rapid blastocyst vitrification protocol compared with the standard approach.

Key Dates

First listed
Apr 29, 2026
Start date
Jun 1, 2026
Status verified
Jun 2026
Primary completion
Dec 31, 2027
Completion
Mar 31, 2028

Study Design

Enrollment
80 participants (estimated)
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT

Arms

  • Active Comparator: Standard Vitrification Protocol
    The standard vitrification protocol corresponds to the routine clinical practice of the laboratory and consists of the following steps: 1. Equilibration step: Blastocysts are equilibrated in Equilibration Solution (ES) for 10-12 minutes at room temperature. 2. Vitrification solution exposure: Blastocysts are then transferred to Vitrification Solution (VS) for a maximum exposure time of 90 seconds. 3. Loading: The blastocyst is loaded onto a Cryotop device (Kitazato). 4. Vitrification: The Cryotop is plunged directly into liquid nitrogen within 1 second of loading to ensure ultra-rapid cooling. 5. Capping: The Cryotop is immediately capped under liquid nitrogen conditions.
  • Experimental: Ultra-Rapid Vitrification Protocol
    The ultra-rapid vitrification protocol is identical to the standard protocol in terms of media, devices, and laboratory conditions, with the following modifications: Preparation step - Blastocyst shrinkage: Prior to equilibration, artificial collapse of the blastocyst is performed using a single laser pulse to induce blastocoel shrinkage. The subsequent steps are: 1\. Equilibration step: Blastocysts are equilibrated in Equilibration Solution (ES) for 2-4 minutes. Vitrification solution exposure: Transfer to Vitrification Solution (VS) for a maximum of 90 seconds. 3\. Loading: Loading of the blastocyst onto a Cryotop device. 4. Vitrification: Immediate plunging of the Cryotop into liquid nitrogen within 1 second. 5\. Capping: Immediate capping under liquid nitrogen.

Primary Outcome Measure

Post-warming survival rate [ Time Frame: Immediately after warming (within the same laboratory session) ]

Central Contacts

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