Single-Cell Insights Into Diabetic Retinopathy Mechanisms

Sponsor
Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
Study ID
NCT06497608
Status
Not Yet Recruiting

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Conditions

Eligibility Criteria

Sex
ALL
Age
18 Years - N/A
Healthy Volunteers
Not accepted

Study Details

This study aims to uncover the cellular and molecular mechanisms of diabetic retinopathy through single-cell sequencing to identify new therapeutic targets. The study will collect retinal tissue samples from 3 patients with advanced proliferative diabetic retinopathy who underwent prosthetic eye implantation and 3 normal cadaver donors. Additionally, aqueous humor, vitreous fluid, and plasma samples from patients with severe diabetic retinopathy requiring surgery and macular hole surgery patients without diabetic retinopathy will be analyzed using ELISA, qPCR, and Western Blot. These experiments will validate sequencing results and explore the pathogenesis of diabetic retinopathy to identify potential treatment targets.

Key Dates

First listed
Jul 12, 2024
Start date
Jul 31, 2024
Status verified
Jul 2024
Primary completion
Sep 30, 2024
Completion
Jul 31, 2027

Study Design

Enrollment
66 participants (estimated)

Arms

  • Arm: scRNA_DR
    Patients with proliferative diabetic retinopathy who underwent prosthetic eye implantation.
  • Arm: scRNA_normal
    Body donors without history of diabetic retinopathy
  • Arm: Validate_DR
    Patients with proliferative diabetic retinopathy (PDR) who underwent vitrectomy surgery
  • Arm: Validate_normal
    Patients who underwent vitrectomy surgery for macular hole (MH) and did not have diabetic retinopathy (DR)

Primary Outcome Measure

Single cell sequencing result [ Time Frame: 1 month ]

Central Contacts

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Diabetic retinopathy trialsOphthalmology trialsRetina trials

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